Real-world conditions impact feed enzyme stability during storage, premixing, and pelleting. In many countries, humid and hot climate conditions can lead to loss of activity in enzyme products. Moreover, if the pH or temperature is too extreme, the enzymes can become inactive, reducing or eliminating their effectiveness in breaking down feed components. Additionally, not all enzyme molecules can withstand the acidic conditions in the stomach of monogastric animals, thereby losing activity before they can hydrolyze the antinutritional factors. If the animals do not receive the intended enzyme dose with their feed due to the factors mentioned above, or if the enzyme is not optimally adapted to the gastrointestinal conditions of the animal, it can lead to inconsistent or even negative effects on animal performance. This paper gives an overview on important parameters, their relevance for feed enzymes in general and characteristics of Natupulse® TS, BASF's new ß-mannanase enzyme
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